Wilson And Blair'S Bbs Agar Medium 10. (As Per Ip) (Triple Pack)

-Dissolve 4.5 grams of Part I in 100 ml purified/ distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Dissolve 5.6 grams of Part II [Bismuth Sulphite Glucose Phosphate Mixture, solution (i)] in 20 ml sterile purified/ distilled water, boil the solution till all the ingredients are properly dissolved. -Dissolve 0.045 grams of Part III [Iron Citrate Brilliant Green Mixture, solution (ii)] in 4.5 ml sterile purified/ distilled water. -Mix aseptically 20 ml of Part II and 4.5 ml of Part III with 100 ml of previously melted sterile Nutrient Agar (Part I) and cool to a temperature 60°C and pour into sterile Petri plates. Note: Directions specified are as per the concurrent edition of pharmacopoeia in force. Specified expiry period corresponds to this. User must ensure its compatibility with the latest edition.

-Dissolve 4.5 grams of Part I in 100 ml purified/ distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Dissolve 5.6 grams of Part II [Bismuth Sulphite Glucose Phosphate Mixture, solution (i)] in 20 ml sterile purified/ distilled water, boil the solution till all the ingredients are properly dissolved. -Dissolve 0.045 grams of Part III [Iron Citrate Brilliant Green Mixture, solution (ii)] in 4.5 ml sterile purified/ distilled water. -Mix aseptically 20 ml of Part II and 4.5 ml of Part III with 100 ml of previously melted sterile Nutrient Agar (Part I) and cool to a temperature 60°C and pour into sterile Petri plates. Note: Directions specified are as per the concurrent edition of pharmacopoeia in force. Specified expiry period corresponds to this. User must ensure its compatibility with the latest edition.