V.C.N.T. Supplement

Rehydrate the contents of 1 vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add it to TM 933 - THAYER MARTIN MEDIUM BASE for 440 ml of medium aseptically add 50ml sterile lysed blood and one vial of V.C.N.T Supplement along with one vial of Vitamino Growth Supplement, 2% Haemoglobin Solution (250ml) can be used instead of sterile lysed blood in 250ml of medium. In TM 116 - GC AGAR BASE / TMV 116 - GC AGAR BASE (VEG.) for 250 ml of medium aseptically add 250 ml of 2% Haemoglobin Solution and GC Supplement w/Antibiotics, one vial of Vitamino Growth Supplement w/ Antibiotics or Yeast Autolysate Supplement. If desired V.C.N.T Supplement can be used along with GC Supplement w/ Antibiotics for additiol selectivity. In TM 1105 - TRANSGROW MEDIUM BASE for 440 ml of medium aseptically add 50 ml of sterile 2% Haemoglobin Solution and one vial of V.C.N.T Supplement along with one vial of Vitamino Growth Supplement.

Rehydrate the contents of 1 vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add it to TM 933 - THAYER MARTIN MEDIUM BASE for 440 ml of medium aseptically add 50ml sterile lysed blood and one vial of V.C.N.T Supplement along with one vial of Vitamino Growth Supplement, 2% Haemoglobin Solution (250ml) can be used instead of sterile lysed blood in 250ml of medium. In TM 116 - GC AGAR BASE / TMV 116 - GC AGAR BASE (VEG.) for 250 ml of medium aseptically add 250 ml of 2% Haemoglobin Solution and GC Supplement w/Antibiotics, one vial of Vitamino Growth Supplement w/ Antibiotics or Yeast Autolysate Supplement. If desired V.C.N.T Supplement can be used along with GC Supplement w/ Antibiotics for additiol selectivity. In TM 1105 - TRANSGROW MEDIUM BASE for 440 ml of medium aseptically add 50 ml of sterile 2% Haemoglobin Solution and one vial of V.C.N.T Supplement along with one vial of Vitamino Growth Supplement.