UREA BROTH BASE (CHRISTENSEN)
-Dissolve 0.9 grams in 95 ml distilled water.
-Heat with frequent agitation to completely dissolve the medium if necessary.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C then add aseptically 5ml of sterile urea solution.
-Dissolve 0.9 grams in 95 ml distilled water.
-Heat with frequent agitation to completely dissolve the medium if necessary.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C then add aseptically 5ml of sterile urea solution.
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