UREA AGAR BASE (CHRISTENSEN) (IS:5887 (Part-I)-1976)
-Dissolve 24.01 grams in 950 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 10 psi pressure (115°C) for 20 minutes.
-Cool to 45-50°C and aseptically add 50 ml of sterile 40% Urea Solution and mix well.
-Dispense into sterile tubes and allow to set in a slanting position. Do not overheat or reheat the medium as urea decomposes very easily.
-Dissolve 24.01 grams in 950 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 10 psi pressure (115°C) for 20 minutes.
-Cool to 45-50°C and aseptically add 50 ml of sterile 40% Urea Solution and mix well.
-Dispense into sterile tubes and allow to set in a slanting position. Do not overheat or reheat the medium as urea decomposes very easily.
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