INTENDED USE
An enzymatic hydrolysate of protein that can replace meat infusion used in the preparation of culture media for the cultivation of fastidious organisms and for cell culture applications.
MLSIGNUP100
Expiring in 23days 3hr7min37sec1521 - TRYPTOSE (Enzymatic Hydrolysate Of Protein That Replace Meat Infusion)
PRODUCT SUMMARY AND EXPLANATION
Tryptose is very useful for cultivation of streptococci, pneumococci, meningococci and other fastidious organisms, and was found to be superior to meat infusion peptone media previously used for these organisms. It has been reported as beneficial for cell culture applications. Tryptose to be suitable for supplementing a serum-free medium to grow human diploid fibroblasts. Tryptose provided free amino acids necessary for growth of Spodoptera frugiperda and Lymantria dispar insect cell lines. Tryptose is often used as a biomass enhancer for recombinant E. coli production.
PRINCIPLE
Tryptose is a mixed enzymatic hydrolysate with distinctive nutritional properties. It is designed to promote good luxuriant growth of highly fastidious microorganisms. Even though enzymically digested, it can be used in place of meat infusion to meet the nutritional requirements of fastidious microorganisms.
QUALITY CONTROL SPECIFICATIONS
Appearance : Creamish to yellowish color, free flowing powder having
characteristic odor but not pungent smell.
Solubility (2% soln. at 25ºC) : Soluble in distilled Water, Clear. Insoluble in alcohol.
Clarity (2% Soln. at 121ºC) : Clear solution. No ppt.
pH (2% Soln. at 25ºC) : 6.5 – 7.5
COMPOSITION
Loss on drying (at 105ºC) | NMT – 6.0% |
Total Nitrogen (DWB) | NLT – 12.0% |
αAmino Nitrogen | NLT – 3.5% |
Total Ash | NMT – 15.0% |
Chloride (as NaCl) | NMT – 6.0% |
Heavy Metals (Pb) | NMT – 20 ppm |
Microbial test | Passes Test |
Growth Promotional Test | Passes Test |
Indole Test | Positive |
INSTRUCTION FOR USE
It is used in the preparation of culture media for the cultivation of many fastidious microorganisms.
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