-Suspend 33 grams in 1000 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool the autoclaved medium to 45 - 50°C and aseptically add 5% v/v sterile defibrited blood.
-Mix thoroughly, avoiding air bubbles and pour into sterile Petri plates.
-Suspend 33 grams in 1000 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool the autoclaved medium to 45 - 50°C and aseptically add 5% v/v sterile defibrited blood.
-Mix thoroughly, avoiding air bubbles and pour into sterile Petri plates.
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