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Triple Sugar Iron Agar
INTENDED USE
For identification of gram-negative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and H2S production.
MLSIGNUP100
Expiring in 0days 6hr6min38sec
TRIPLE SUGAR IRON AGAR
PRODUCT SUMMARY AND EXPLANATION
Triple Sugar Iron Agar was originally proposed by Sulkin and Willett and modified by Hajna for identifying Enterobacteriaceae. This medium complies with the recommendation of Indian Pharmacopoeia for the identification of
Gram-negative bacilli.
PRINCIPLE
Peptone, yeast extract and beef extract provide nitrogenous compounds sulphur, trace elements and vitamin B complex etc. Sodium chloride maintains osmotic equilibrium. Lactose, sucrose and dextrose monohydrate are the fermentable carbohydrates. Sodium thiosulphate and ferric or ferrous ions make H2S indicator system. Sodium thiosulphate is also an inactivator of halogen and can minimize its toxicity in the testing sample, if any during microbial limit tests. Phenol red is the pH indicator.
QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Light yellow to pink homogeneous free flowing powder.
Appearance of prepared medium : Pinkish red coloured clear to slightly opalescent gel forms in Petri plates.
pH (at 25°C) : 7.4±0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Beef extract | 3.000 |
| Peptone | 20.000 |
| Yeast extract | 3.000 |
| Lactose | 10.000 |
| Sucrose | 10.000 |
| Dextrose monohydrate | 1.000 |
| Ferrous sulphate | 0.200 |
| Sodium chloride | 5.000 |
| Sodium thiosulphate | 0.300 |
- Dissolve 64.42 grams in 1000 ml distilled water.
- Heat to boiling to dissolve the medium completely.
- Mix well and distribute in test tubes.
- Sterilize by autoclaving at 15 lbs pressure (115°C) for 30 minutes or as per validated cycle. Allow the medium to set
in sloped form with a butt about 2.5cm long.
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