TRIPLE SUGAR IRON AGAR (as per EP)
-Dissolve 64.42 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/ distilled water.
-Heat to boiling to dissolve the medium completely.
-Mix well and distribute into test tubes and Sterilize by maintaining at 10 psi pressure (115°C) for 30 minutes or as per validated cycle.
-Allow the medium to set in sloped form with a butt about 2.5cm long.
-Note: Directions specified are as per the concurrent edition of pharmacopoeia in force. Specified expiry period corresponds to this.
-Dissolve 64.42 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/ distilled water.
-Heat to boiling to dissolve the medium completely.
-Mix well and distribute into test tubes and Sterilize by maintaining at 10 psi pressure (115°C) for 30 minutes or as per validated cycle.
-Allow the medium to set in sloped form with a butt about 2.5cm long.
-Note: Directions specified are as per the concurrent edition of pharmacopoeia in force. Specified expiry period corresponds to this.
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