TRIBUTYRIN AGAR BASE W/O TRIBUTYRIN
-Suspend 23 grams in 990 ml purified / distilled water.
-Add 10 ml of Tributyrin. Mix and heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C.
-Mix well and pour into sterile Petri plates.
-Suspend 23 grams in 990 ml purified / distilled water.
-Add 10 ml of Tributyrin. Mix and heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C.
-Mix well and pour into sterile Petri plates.
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