-Suspend 80.05 grams in 1000 ml distilled water, preheated to a temperature of 50°C.
-Mix well and allow to stand for 5 minutes. Heat to boiling to dissolve the medium completely.
-Dispense in test tubes filling them upto half of the tubes. Sterilize by autoclaving at 118°C for 15 minutes.
-Suspend 80.05 grams in 1000 ml distilled water, preheated to a temperature of 50°C.
-Mix well and allow to stand for 5 minutes. Heat to boiling to dissolve the medium completely.
-Dispense in test tubes filling them upto half of the tubes. Sterilize by autoclaving at 118°C for 15 minutes.
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