-Suspend 21.0 grams in 450 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45°C.
-Aseptically add 50 ml of sterile lysed blood and rehydrated content
-Suspend 21.0 grams in 450 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45°C.
-Aseptically add 50 ml of sterile lysed blood and rehydrated content
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