TETRATHIONATE BROTH BASE, HAJNA (TT BROTH BASE)
-Suspend 91.51 grams in 1000 ml distilled water.
-Heat just to boiling or place in flowing steam for 30 minutes. DO NOT AUTOCLAVE.
-Cool to 45°C. Mix and add 40 ml of Iodine solution (8 g potassium iodide and 5 g iodine per 40 ml). Mix and dispense 10 ml amounts in tubes. Do not heat after addition of iodine.
-Suspend 91.51 grams in 1000 ml distilled water.
-Heat just to boiling or place in flowing steam for 30 minutes. DO NOT AUTOCLAVE.
-Cool to 45°C. Mix and add 40 ml of Iodine solution (8 g potassium iodide and 5 g iodine per 40 ml). Mix and dispense 10 ml amounts in tubes. Do not heat after addition of iodine.
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