TETRATHIONATE BROTH BASE (as per IP)
-Suspend 77.40 grams in 980 ml purified/ distilled water.
-Heat just to boiling. Cool below 45-50°C and aseptically add 20 ml iodine solution (6 grams of iodine and 5 grams of potassium iodide in 20 ml distilled water).
-Mix well and dispense in 10 ml quantities in sterile tubes. This complete medium should be used on the day of preparation. Do not heat after the addition of iodine solution.
-Use the medium immediately after addition of iodine. Note: Due to presence of calcium carbote, the prepared medium forms opalescent solution with a white precipitate.
-Suspend 77.40 grams in 980 ml purified/ distilled water.
-Heat just to boiling. Cool below 45-50°C and aseptically add 20 ml iodine solution (6 grams of iodine and 5 grams of potassium iodide in 20 ml distilled water).
-Mix well and dispense in 10 ml quantities in sterile tubes. This complete medium should be used on the day of preparation. Do not heat after the addition of iodine solution.
-Use the medium immediately after addition of iodine. Note: Due to presence of calcium carbote, the prepared medium forms opalescent solution with a white precipitate.
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