SULPHATE REDUCING MEDIUM (TRIPLE PACK) (PART - I)
-Dissolve 7.10 grams of Part I in 900 ml distilled water. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. On the day of use prepare solution of Part II by suspending 0.49 grams of Part II in 100 ml distilled water. -Sterilize by filtration through a 0.45 µm membrane filter and aseptically add this 100 ml solution to 900 ml Part I medium. -Then separately sterilize the 3.50 grams Part III by autoclaving at 15 psi pressure (121°C) for 15 minutes and aseptically add to the mixture of Part I and II. -Mix well and aseptically transfer the complete medium to sterile screw capped tubes filling them completely.
-Dissolve 7.10 grams of Part I in 900 ml distilled water. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. On the day of use prepare solution of Part II by suspending 0.49 grams of Part II in 100 ml distilled water. -Sterilize by filtration through a 0.45 µm membrane filter and aseptically add this 100 ml solution to 900 ml Part I medium. -Then separately sterilize the 3.50 grams Part III by autoclaving at 15 psi pressure (121°C) for 15 minutes and aseptically add to the mixture of Part I and II. -Mix well and aseptically transfer the complete medium to sterile screw capped tubes filling them completely.
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