STUART TRANSPORT MEDIUM W/O METHYLENE BLUE WITH CHARCOAL
-Dissolve 24 grams in 1000 ml double distilled water.
-Heat to boiling to dissolve the medium completely. Dispense into tubes with screw caps to give a depth of approximately 7 cm.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes and after sterilization, tighten the caps.
-Cool in an upright position. Turn the tubes several times while agar is solidifying, to maintain uniform suspension of charcoal particles.
Note: Care should be taken that the water is free from chlorine.
-Dissolve 24 grams in 1000 ml double distilled water.
-Heat to boiling to dissolve the medium completely. Dispense into tubes with screw caps to give a depth of approximately 7 cm.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes and after sterilization, tighten the caps.
-Cool in an upright position. Turn the tubes several times while agar is solidifying, to maintain uniform suspension of charcoal particles.
Note: Care should be taken that the water is free from chlorine.
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