SALT POLYMYXIN BROTH BASE (as per ISO 8914 : 1990)
-Dissolve 29.70 grams in 1000ml purified/distilled water.
-Gently heat to boiling with swirling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121oC) for 15 minutes.
-Cool to 45-50 oC.
-Aseptically add rehydrated contents of 2 vial of Polymyxin B Selective supplement (TS 058).
-Mix well and dispense into sterile tubes or flasks as desired.
-Dissolve 29.70 grams in 1000ml purified/distilled water.
-Gently heat to boiling with swirling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121oC) for 15 minutes.
-Cool to 45-50 oC.
-Aseptically add rehydrated contents of 2 vial of Polymyxin B Selective supplement (TS 058).
-Mix well and dispense into sterile tubes or flasks as desired.
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