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Triple Sugar Iron Agar Slant (10 Slants)
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TRIPLE SUGAR IRON AGAR SLANT (10 Slants)
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Triple Sugar Iron Agar Slant (10 Slants)
1. Using freshly grown bacterial cultures, inoculate the tubes with an inoculating needle by stabbing the butt
and streaking back and forth along the surface of the slant.
2. Incubate tubes with loosened caps at 35 ± 2 °C in an aerobic atmosphere.
3. Examine tubes after 18–24 h for growth and reactions.
1. Using freshly grown bacterial cultures, inoculate the tubes with an inoculating needle by stabbing the butt
and streaking back and forth along the surface of the slant.
2. Incubate tubes with loosened caps at 35 ± 2 °C in an aerobic atmosphere.
3. Examine tubes after 18–24 h for growth and reactions.
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