-Dissolve 24.20 grams in 500 ml purified/distilled water containing 5 ml glycerol.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add sterile rehydrated contents of either CetriNix Supplement or CFC Supplement as desired.
-Mix well and pour into sterile Petri plates.
-Dissolve 24.20 grams in 500 ml purified/distilled water containing 5 ml glycerol.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add sterile rehydrated contents of either CetriNix Supplement or CFC Supplement as desired.
-Mix well and pour into sterile Petri plates.
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