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Potato Dextrose Broth

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TM 331
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INTENDED USE

For cultivation and enumeration of yeasts and molds.

MLSIGNUP100

Expiring in 18days 15hr8min1sec

POTATO DEXTROSE BROTH

PRODUCT SUMMARY AND EXPLANATION
Potato Dextrose Broth is recommended by APHA and F.D.A. for plate counts of yeasts and moulds in the examination of foods and dairy products. Potato Dextrose Broth is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production.

PRINCIPLE
This medium consists of Potato infusion and dextrose that promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided.

QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Off-white to yellow homogeneous free flowing powder.
Appearance of prepared medium : Light amber coloured clear to slightly opalescent solution in tubes.
pH (at 25°C) : 5.1 ± 0.2

 

COMPOSITION

Ingredients  Gms / Ltr
Potatoes, infusion from  200.000
Dextrose (Glucose)  20.000

INSTRUCTION FOR USE

  • Dissolve 24.0 grams in 1000 ml purified / distilled water.
  • Heat if necessary to dissolve the medium completely.
  • Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
  • Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.
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