-Dissolve 39 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121° C) for 15 minutes.
-Mix well before dispensing.
-In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml of sterile, cooled medium is approximately 1 ml. DO NOT HEAT the medium after addition of the acid.
-Dissolve 39 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121° C) for 15 minutes.
-Mix well before dispensing.
-In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml of sterile, cooled medium is approximately 1 ml. DO NOT HEAT the medium after addition of the acid.
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