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Plate Count Agar (Standard Plate Agar)
INTENDED USE
For determination of plate counts of microorganisms in foods, water, waste water and also from clinical samples.
MLSIGNUP100
Expiring in 29days 21hr6min27sec
PLATE COUNT AGAR (STANDARD PLATE AGAR)
PRODUCT SUMMARY AND EXPLANATION
Plate count agar is used for determination of plate counts of microorganisms from samples. This media was formulated and described by Buchbinder et al. Plate count agar is also suitable for determining bacterial count in food and water, indicating microbial contamination. This culture medium complies with the specifications given by ISO 4833-1 & 2:2013 and APHA.
PRINCIPLE
The medium consists of Tryptone which provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly the B-group. Dextrose is the fermentable sugar providing carbon and energy. Agar acts as a solidifying agent.
QUALITY CONTROL SPECIFICATIONS
Appearance of Dehydrated powder : Cream to yellow, homogeneous free flowing powder.
Appearance of Prepared medium : Light yellow colored, clear to slightly opalescent gel.
pH (at 25°C) : 7.0± 0.2
COMPOSITION
| Ingredients | Gms / Ltr |
| Agar | 15.000 |
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose | 1.000 |
INSTRUCTION FOR USE
- Dissolve 23.50 grams in 1000ml distilled water
- Gently heat to boiling with gentle swirling to dissolve the medium completely
- Sterilize by autoclaving at 15 psi (121°C) for 15 minute
Cool to 45-50°C - Mix well and pour into sterile Petri plates.
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