PIKE STREPTOCOCCAL BROTH BASE
-Dissolve 30.26 grams in 1000 ml distilled water.
-Heat if necessary to dissolve the medium completely.
-Dispense in 100 ml amounts in flasks. Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 5% v/v sterile defibrited rabbit blood.
-Mix well and dispense aseptically in 2 ml amounts in sterile tubes.
Warning: Sodium azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.
-Dissolve 30.26 grams in 1000 ml distilled water.
-Heat if necessary to dissolve the medium completely.
-Dispense in 100 ml amounts in flasks. Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 5% v/v sterile defibrited rabbit blood.
-Mix well and dispense aseptically in 2 ml amounts in sterile tubes.
Warning: Sodium azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.
The information below is required for social login
Sign In
Create New Account