PHENOL RED BROTH BASE W/ MEAT EXTRACT
-Dissolve 16.02 grams in 1000 ml purified/distilled water. Add the test carbohydrate in desired quantity.
-Heat if necessary to dissolve the medium completely.
-Mix well and dispense in fermentation tubes (tubes containing inverted Durham's tubes).
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
Note: For critical studies, it is recommended to use filter sterilized carbohydrate which is to be incorporated aseptically in the sterile medium base, if desired.
-Dissolve 16.02 grams in 1000 ml purified/distilled water. Add the test carbohydrate in desired quantity.
-Heat if necessary to dissolve the medium completely.
-Mix well and dispense in fermentation tubes (tubes containing inverted Durham's tubes).
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
Note: For critical studies, it is recommended to use filter sterilized carbohydrate which is to be incorporated aseptically in the sterile medium base, if desired.
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