-Dissolve 16.02 grams in 1000 ml purified/distilled water, mix well.
-Heat if necessary to dissolve the medium completely.
-Mix well and dispense in fermentation tubes (tubes containing inverted Durham's tubes).
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Aseptically add filter sterilized or autoclave sterilized carbohydrate solution to sterile basal medium.
-Dissolve 16.02 grams in 1000 ml purified/distilled water, mix well.
-Heat if necessary to dissolve the medium completely.
-Mix well and dispense in fermentation tubes (tubes containing inverted Durham's tubes).
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Aseptically add filter sterilized or autoclave sterilized carbohydrate solution to sterile basal medium.
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