-Dissolve 31.02 grams in 1000 ml purified/distilled water.
-Add 5-10 grams of carbohydrate as desired.
-Heat to boiling to dissolve the medium completely.
-Dispense in tubes or flasks as desired and sterilize by autoclaving at 15 psi pressure (121° C) for 15 minutes.
-Allow the tubed media to cool in slanted position to form slants with deep butts.
Note: For critical studies, it is recommended to use filter sterilized carbohydrate which is to be incorporated aseptically in sterile medium base.
-Dissolve 31.02 grams in 1000 ml purified/distilled water.
-Add 5-10 grams of carbohydrate as desired.
-Heat to boiling to dissolve the medium completely.
-Dispense in tubes or flasks as desired and sterilize by autoclaving at 15 psi pressure (121° C) for 15 minutes.
-Allow the tubed media to cool in slanted position to form slants with deep butts.
Note: For critical studies, it is recommended to use filter sterilized carbohydrate which is to be incorporated aseptically in sterile medium base.
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