Perfringen’S T.S.C.Supplement (Tsc Supplement)

Rehydrate the contents of 1 vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add to 475 ml of sterile, molten, cooled (45-50°C) TM 615 - PERFRINGENS AGAR BASE (T.S.C./S.F.P. AGAR BASE) / TMV 615 - PERFRINGENS AGAR BASE (T.S.C./S.F.P. AGAR BASE) (VEG.) / TM 2311 - S.F.P. AGAR BASE along with 25 ml of Egg Yolk Emulsion or 500 ml of sterile, molten, cooled (45-50°C) TM 1826 - PERFRINGENS AGAR BASE (TRYPTOSE SULPHITE CYCLOSERINE AGAR BASE) (ISO 7937: 2004, ISO 14189:2013) / TM 809 - TRYPTOSE CYCLOSERINE AZIDE AGAR BASE / TM 902 - TRYPTOSE CYCLOSERINE DEXTROSE AGAR BASE / TM 2400 - TRYPTONE YEAST SODIUM SULPHITE AGAR BASE (ISO 14189:2013). Mix well and pour into sterile petri plates.

Rehydrate the contents of 1 vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add to 475 ml of sterile, molten, cooled (45-50°C) TM 615 - PERFRINGENS AGAR BASE (T.S.C./S.F.P. AGAR BASE) / TMV 615 - PERFRINGENS AGAR BASE (T.S.C./S.F.P. AGAR BASE) (VEG.) / TM 2311 - S.F.P. AGAR BASE along with 25 ml of Egg Yolk Emulsion or 500 ml of sterile, molten, cooled (45-50°C) TM 1826 - PERFRINGENS AGAR BASE (TRYPTOSE SULPHITE CYCLOSERINE AGAR BASE) (ISO 7937: 2004, ISO 14189:2013) / TM 809 - TRYPTOSE CYCLOSERINE AZIDE AGAR BASE / TM 902 - TRYPTOSE CYCLOSERINE DEXTROSE AGAR BASE / TM 2400 - TRYPTONE YEAST SODIUM SULPHITE AGAR BASE (ISO 14189:2013). Mix well and pour into sterile petri plates.