MYP AGAR BASE (PHENOL RED EGG YOLK POLYMYXIN AGAR BASE)
-Dissolve 46.02 grams in 900ml distilled water.
-Gently heat to boiling with swirling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121°C) for 15 minutes
-Cool to 45-50°C and aseptically add 2 vials of POLYMYXIN B SELECTIVE SUPPLEMENT (TS 058) and 100ml EGG YOLK EMULSION (TS 002) per 900ml of medium.
-Mix well and pour into petri plates.
-Dissolve 46.02 grams in 900ml distilled water.
-Gently heat to boiling with swirling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi (121°C) for 15 minutes
-Cool to 45-50°C and aseptically add 2 vials of POLYMYXIN B SELECTIVE SUPPLEMENT (TS 058) and 100ml EGG YOLK EMULSION (TS 002) per 900ml of medium.
-Mix well and pour into petri plates.
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