-Dissolve 36.0 grams in 700 ml purified/ distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 300 ml Horse serum or 10 vials of
-Dissolve 36.0 grams in 700 ml purified/ distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 300 ml Horse serum or 10 vials of
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