Mueller Hinton Broth (Veg.)

TMV 325 - MUELLER HINTON BROTH (VEG.)

PRODUCT SUMMARY AND EXPLANATION
These media are prepared by completely replacing animal based peptones with vegetable peptones. Mueller Hinton Veg Broth is the modification of Mueller Hinton Broth which is recommended for the diffusion of antimicrobial agents
impregnated on paper disc through an agar gel as described in CLSI Approved Standard. Mueller Hinton Veg Broth is used for determining Minimal Inhibitory Concentration (MIC) of antimicrobials for aerobic bacteria. Kirby-Bauer et al
recommended this medium for performing antibiotic susceptibility tests using a single disc of high concentration. A standardized suspension of the organisms is swabbed over the entire surface of the agar medium. Paper discs impregnated with certain amount of specific antibiotics are placed on the surface of the medium. The plates are incubated and the zones of inhibition around each disc are measured. It is then determined whether the organism is susceptible, intermediate or resistant to an agent by comparing the zone-sizes to standard zone-sizes. Different factors influence the disc diffusion susceptibility tests as, inoculum concentration, agar depth, disc potency, medium pH and beta-lactamase production by test organisms.

PRINCIPLE
Veg infusion and Veg acid hydrolysate provide nitrogenous compounds, carbon, sulphur and other essential nutrients. Starch acts as a “protective colloid” against toxic substances present in the medium. During autoclaving the starch gets hydrolyzed and provides some amount of dextrose, which then serves as energy source. Growth of Gonococci and Meningococci is highly satisfactory on this medium.

QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Cream to yellow homogeneous free flowing powder.
Appearance of prepared medium : Light amber coloured clear solution in tubes.
pH (at 25°C) : 7.3±0.2