Mueller Hinton Broth

TM 325 - MUELLER HINTON BROTH

PRODUCT SUMMARY AND EXPLANATION
Mueller Hinton Broth is a stable serum-free bacteriological media that supported the growth of two otherwise very fastidious bacteria, the meningococcus and the gonococcus. Later it was improved by supplementing cations to enhance
Pseudomonas recovery and for propagating the vast majority of pathogenic bacteria encountered in clinical practice. In the beginning of the antibiotic era, the versatility of Mueller Hinton Broth and its agar was a perfect match, allowing a standardized, consistent medium for broth dilution or disk diffusion testing of antibiotic potency, defined most frequently in terms of minimum inhibitory concentration (MIC). In recent decades, MHB has been endorsed by the Clinical and Laboratory Standards Institute (CLSI), the global nonprofit organization that ensures quality in healthcare testing, as the appropriate media for routine bacterial antibiotic susceptibility determination, with updated cutoff standards for designating resistant and susceptible strains.

PRINCIPLE
Beef, infusion form and Casein acid hydrolysate provide nitrogenous compounds, carbon, sulphur and other essential nutrients. Starch acts as a protective colloid against toxic substances present in the medium. These ingredients are selected for low thymine and thymidine content as determined by MIC values for Enterococcus faecalis with sulfamethoxazole trimethoprim (SXT). The CLSI recommend the following cation levels Ca++, 20-25mg/liter; Mg++, 10-12.5mg/liter to be present in the broth while performing broth dilution method. Cation level is usually inadequate in the
medium; the concentration of cations already present in the broth must the amount of Ca++ or Mg ++; if that needs to be added. Cation stock for addition of supplemental cations are recommended by CLSI which is 8.36g of MgCl2.6H2O in 100ml contains 10mg of Mg++/ ml and 3.68g of CaCl2.2Hin2O100ml contains 10 mg of Ca++/ml. Alternatively, Mueller Hinton
Broth No. 2 Control Cations is available wherein cations are adjusted as per Standard guidelines.