Middlebrook Adc Growth Supplement
Warm up the refrigerated supplement to 45-50ºC. Shake well to form uniform suspension. Aseptically add 1 vial to 450
ml of sterile, molten, cooled (45-50°C) TM 213 - MIDDLEBROOK 7H9 BROTH BASE. Mix well and pour into sterile screw
capped tubes.
Warm up the refrigerated supplement to 45-50ºC. Shake well to form uniform suspension. Aseptically add 1 vial to 450
ml of sterile, molten, cooled (45-50°C) TM 213 - MIDDLEBROOK 7H9 BROTH BASE. Mix well and pour into sterile screw
capped tubes.
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