Polycarbonate Jar

1. Place the petri dishes in the carrier which should be of the vented variety to aid gas transfer between the interior and exterior of the plates. 2. Insert Aerobic Indicator Tablet into the smaller (upper) clip in the plate carrier 3. Put the loaded carrier into the polycarbote jar 4. Place the lid fitted with accessories in the jar after making sure that the silicon ‘O’- ring is correctly placed on the jar. Apply the three finger clamp and screw down until tight. 5. A metal accessory med as vacuum chuck have to be used for the Evacuation/ Replacement technique to eble first vacuum to be down. 6. Fit the vacuum chuck connected to the vacuum line to the valve marked as ‘vacuum’ and press (not screw). The open end of the chuck firmly down on to the vacuum valve body. Screwing will damage the sealing rubber washer and cause the chuck to leak. 7. Evacuate the system to about -30 mm of Hg. 8. After use, simply lift the vacuum chuck straight away from the vacuum valve in order to disconnect it. Observe the pressure gauge. A leakage in the jar will be detected at this stage because the vacuum reading will not remain constant. 9. Attach the pressure chuck connected to the gas supply to the pressure valve of the jar. Run the gas mixture into jar until pressure is zero. Disconnect the pressure chuck. 10. Observe pressure changes in the jar. 11. Release more gas mixture to the jar until the gauge reads zero. Disconnect the pressure chuck. Incubate the jar. 12. The Aerobic Indicator tablet will remain pink in the jar indicating aerobiosis. Any kind of leakage leading to aerobic conditions will turn the colour of the tablets to purple. 13. The interior of the lid and jar should be kept free of dust.

1. Place the petri dishes in the carrier which should be of the vented variety to aid gas transfer between the interior and exterior of the plates. 2. Insert Aerobic Indicator Tablet into the smaller (upper) clip in the plate carrier 3. Put the loaded carrier into the polycarbote jar 4. Place the lid fitted with accessories in the jar after making sure that the silicon ‘O’- ring is correctly placed on the jar. Apply the three finger clamp and screw down until tight. 5. A metal accessory med as vacuum chuck have to be used for the Evacuation/ Replacement technique to eble first vacuum to be down. 6. Fit the vacuum chuck connected to the vacuum line to the valve marked as ‘vacuum’ and press (not screw). The open end of the chuck firmly down on to the vacuum valve body. Screwing will damage the sealing rubber washer and cause the chuck to leak. 7. Evacuate the system to about -30 mm of Hg. 8. After use, simply lift the vacuum chuck straight away from the vacuum valve in order to disconnect it. Observe the pressure gauge. A leakage in the jar will be detected at this stage because the vacuum reading will not remain constant. 9. Attach the pressure chuck connected to the gas supply to the pressure valve of the jar. Run the gas mixture into jar until pressure is zero. Disconnect the pressure chuck. 10. Observe pressure changes in the jar. 11. Release more gas mixture to the jar until the gauge reads zero. Disconnect the pressure chuck. Incubate the jar. 12. The Aerobic Indicator tablet will remain pink in the jar indicating aerobiosis. Any kind of leakage leading to aerobic conditions will turn the colour of the tablets to purple. 13. The interior of the lid and jar should be kept free of dust.