Place one ONPG disc in a sterile test tube. Add 0.1 ml of sterile 0.85% w/v sodium chloride solution (physiological saline). Pick up the colony under test with a sterile loop and emulsify it in physiological saline in the tube containing the disc. Incubate at 35-37°C. To detect active lactose fermenters, observe the tube at an interval of one hour, for upto 6 hours. To detect late lactose fermenters, incubate the tubes for upto 24 hours
Place one ONPG disc in a sterile test tube. Add 0.1 ml of sterile 0.85% w/v sodium chloride solution (physiological saline). Pick up the colony under test with a sterile loop and emulsify it in physiological saline in the tube containing the disc. Incubate at 35-37°C. To detect active lactose fermenters, observe the tube at an interval of one hour, for upto 6 hours. To detect late lactose fermenters, incubate the tubes for upto 24 hours
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