Recommended for the selective isolation of Clostridium difficile
Rehydrate the contents of one vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add to 500 ml sterile, molten, cooled (45-50°C) TM2043 - CLOSTRIDIUM DIFFICILE MANNITOL TAUROCHOLATE BROTH BASE. Mix well and pour into sterile petri plates. Sheep blood may be used in place of horse blood but some strains of the organism will show a slightly reduced growth.
Rehydrate the contents of one vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add to 500 ml sterile, molten, cooled (45-50°C) TM2043 - CLOSTRIDIUM DIFFICILE MANNITOL TAUROCHOLATE BROTH BASE. Mix well and pour into sterile petri plates. Sheep blood may be used in place of horse blood but some strains of the organism will show a slightly reduced growth.
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