-Dissolve 21.67 grams in 1000 ml purified/ distilled water.
-Heat the medium to dissolve completely, if necessary. Do not autoclave.
-Cool to 45-50°C, aseptically add desired quantity (2 to 5 ml) of broth on sterile absorbent cotton pad for saturation. The medium should be used within 24 hours of rehydration.
-Dissolve 21.67 grams in 1000 ml purified/ distilled water.
-Heat the medium to dissolve completely, if necessary. Do not autoclave.
-Cool to 45-50°C, aseptically add desired quantity (2 to 5 ml) of broth on sterile absorbent cotton pad for saturation. The medium should be used within 24 hours of rehydration.
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