M-Broth

M-BROTH 

PRODUCT SUMMARY AND EXPLANATION
Salmonella is facultative anaerobic gram negative bacilli with its typical characteristic oxidase and lactose negative, H2S positive with production of gas. Salmonella resides in intestinal tract of many animals which includes both wild and domestic. Diseases caused by Salmonella in human called Salmonellosis, that includes bacteremia (enteric fever), diarrheal diseases. The severity of diarrheal diseases is directly proportional to virulence of the strain and state oh human source. Agglutination test is one of the serological test which used in the diagnosis to confirm identification. Sperber and Diebel developed M-broth which accelerated the detection of the Salmonella. It functions of providing nutrients and flagella development. The accelerated 50- hour detection includes 18 hours’ pre-enrichment, 24 hours’ selective enrichment, 6-8 hours’ selective enrichment and 2 hours of serological testing. In selective enrichment step Sperber and Diebel has modifies APT Broth by removing dextrose and adding mannose to avoid nonspecific binding. Fantasia et al discovered that enrichment serology method is rapid and less complicated than Bacteriological Analytical Manual to perform. It maintains the sensitivity and accuracy. For isolation and identification of food-borne Salmonella, M-Broth conforms the testing standards which is recommended by the APHA for isolation and identification of foodborne Salmonella.

PRINCIPLE
Organic nitrogen, sulphur, vitamins, carbon, nitrogen provided by the Tryptone and yeast extract, which supports the growth of Salmonella. Sodium chloride maintains the osmotic balance. Mannose act as fermentable sugar and energy
source, this function prevents the fimbrial agglutination of Salmonella. Polysorbate 80 supplies fatty acid, bacterial growth stimulated by the inorganic salts. Dipotassium hydrogen sulphate acts as buffer. 10% suspension is prepared from the sample, which is further prepared in sterile Lactose Broth and incubation temperature is 35±2°C for 18-24 hours. 9ml of Tetrathionate Broth Selenite Cystine broth is added with 1ml of preenriched culture. The enriched culture is inoculated in M-Broth subsequently and incubated at 35±2°C for 6-8 hours followed by the H-agglutination test as per standard procedures.

QUALITY CONTROL SPECIFICATIONS
Appearance of Powder : Cream to yellow, homogenous free flowing powder
Appearance of prepared medium : Light amber colored clear to slightly opalescent solution with slight precipitate.
pH (at 25°C) : 7.0 ± 0.2