LYSINE IRON BROTH BASE (LYSINE IRON CYSTEINE BROTH BASE)
-Dissolve 25.7 grams in 1000 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to room temperature and aseptically add one vial of reconstituted Novobiocin Selective Supplement.
-Mix well before dispensing in sterile tubes or flasks as desired.
-Dissolve 25.7 grams in 1000 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to room temperature and aseptically add one vial of reconstituted Novobiocin Selective Supplement.
-Mix well before dispensing in sterile tubes or flasks as desired.
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