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Lowenstein Jensen Medium Base (L. J. Medium)
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MLSIGNUP100
Expiring in 5days 20hr6min38sec
LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM)
-Dissolve 37.24 grams in 600 ml purified / distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organisms additions of glycerol is not desirable).
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C.
-Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add and mix egg emulsion base and Gruft Mycobacterial Supplement (if desired) gently to obtain uniform mixture.
-Distribute in sterile screw capped tubes. Arrange tubes in a slanted position. Coagulate and inspissate the medium in an inspissator water bath or autoclave at 85°C for 45 minutes.
-Dissolve 37.24 grams in 600 ml purified / distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organisms additions of glycerol is not desirable).
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C.
-Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add and mix egg emulsion base and Gruft Mycobacterial Supplement (if desired) gently to obtain uniform mixture.
-Distribute in sterile screw capped tubes. Arrange tubes in a slanted position. Coagulate and inspissate the medium in an inspissator water bath or autoclave at 85°C for 45 minutes.
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