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Lowenstein Jensen Medium Base (L. J. Medium) (As Per Ip) (Double Pack)
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MLSIGNUP100
Expiring in 11days 20hr6min44sec
LOWENSTEIN JENSEN MEDIUM BASE (L. J. MEDIUM) (as per IP) (DOUBLE PACK)
-Dissolve 6.84 grams of Part I in 600 ml distilled water containing glycerol.
-Sterilize by heating at 121°C for 25 minutes.
-Dissolve 0.4 grams of Part II in 20 ml sterile distilled water under aseptic precautions, allowing the dye to dissolve by incubating for 1 to 2 hours at 37°C. Shake the solution before use.
-Meanwhile prepare 1000 ml of egg emulsion solution collected aseptically. To this add Part I and Part II solution aseptically.
-Distribute 5 ml aliquot into 25 ml McCartney bottles and screw the caps tightly. Lay the bottles horizontally. Coagulate and inspissate the medium in an inspissator or hot air oven at 85°C for 60 minutes.
-Dissolve 6.84 grams of Part I in 600 ml distilled water containing glycerol.
-Sterilize by heating at 121°C for 25 minutes.
-Dissolve 0.4 grams of Part II in 20 ml sterile distilled water under aseptic precautions, allowing the dye to dissolve by incubating for 1 to 2 hours at 37°C. Shake the solution before use.
-Meanwhile prepare 1000 ml of egg emulsion solution collected aseptically. To this add Part I and Part II solution aseptically.
-Distribute 5 ml aliquot into 25 ml McCartney bottles and screw the caps tightly. Lay the bottles horizontally. Coagulate and inspissate the medium in an inspissator or hot air oven at 85°C for 60 minutes.
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