LOEFFLER SERUM MEDIUM BASE
-Dissolve 10.0 grams in 250 ml purified/distilled water at a temperature of 45°C. Add 750 ml of sterile Bovine serum, mix well and dispense into tubes.
-Coagulate and sterilize by inspissation for 15 minutes at 80 to 90°C or steaming at 100°C for 10-15 minutes.
-Dissolve 10.0 grams in 250 ml purified/distilled water at a temperature of 45°C. Add 750 ml of sterile Bovine serum, mix well and dispense into tubes.
-Coagulate and sterilize by inspissation for 15 minutes at 80 to 90°C or steaming at 100°C for 10-15 minutes.
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