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Letheen Broth I Modified
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MLSIGNUP100
Expiring in 1days 9hr6min32sec
LETHEEN BROTH I MODIFIED
-Dissolve 37.46 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. AVOID OVERHEATING.
-Cool to 50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of the medium.
-Mix well before pouring into sterile Petri plates.
Precaution: Store the medium away from light to avoid photo-oxidation.
-Dissolve 37.46 grams in 1000 ml purified/distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. AVOID OVERHEATING.
-Cool to 50°C and shake the medium in order to oxidize the methylene blue (i.e. restore its blue colour) and to suspend the precipitate, which is an essential part of the medium.
-Mix well before pouring into sterile Petri plates.
Precaution: Store the medium away from light to avoid photo-oxidation.
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