Legionella Growth Supplement (Bcye)
Warm up the refrigerated supplement to 45-50ºC, shake well to form uniform suspension. Avoid frothing. Aseptically add
to 440 ml sterile, molten, cooled (45-50°C) TM 1218 - LEGIONELLA AGAR BASE / TM 2156 - LEGIONELLA AGAR BASE W/O
CHARCOAL. If desired aseptically add rehydrated contents of 1 vial of Legionella (GVPC) Selective Supplement. Mix well
and pour into sterile petri plates. The fil pH of the medium will be 6.9 ± 0.2. In case of non-incorporation of Legionella
(GVPC) Selective Supplement, add aseptically 10 ml sterile distilled water to bring the total volume to 500 ml medium.
Warm up the refrigerated supplement to 45-50ºC, shake well to form uniform suspension. Avoid frothing. Aseptically add
to 440 ml sterile, molten, cooled (45-50°C) TM 1218 - LEGIONELLA AGAR BASE / TM 2156 - LEGIONELLA AGAR BASE W/O
CHARCOAL. If desired aseptically add rehydrated contents of 1 vial of Legionella (GVPC) Selective Supplement. Mix well
and pour into sterile petri plates. The fil pH of the medium will be 6.9 ± 0.2. In case of non-incorporation of Legionella
(GVPC) Selective Supplement, add aseptically 10 ml sterile distilled water to bring the total volume to 500 ml medium.
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