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Lactose Monohydrate Broth (Broth Medium D)
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MLSIGNUP100
Expiring in 0days 12hr6min16sec
LACTOSE MONOHYDRATE BROTH (BROTH MEDIUM D)
-Dissolve 12.75 grams in 1000 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely. For larger inocula (10 ml or more), concentrated medium may be prepared to account for medium dilution by the inoculum.
-Dispense in tubes containing inverted fermentation vial (Durham’s tube) as desired.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes and cool immediately.
-Dissolve 12.75 grams in 1000 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely. For larger inocula (10 ml or more), concentrated medium may be prepared to account for medium dilution by the inoculum.
-Dispense in tubes containing inverted fermentation vial (Durham’s tube) as desired.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes and cool immediately.
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