L.MONO CONFIRMATORY AGAR BASE
-Dissolve 38.5 grams in 470 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C. -Aseptically add sterile rehydrated contents of 1 vial of L.Mono Selective Supplement I and 1 vial of L.Mono Selective Supplement II. For enrichment, add sterile contents of 1 vial of L.Mono Enrichment Supplement II. -Mix well and pour into sterile Petri plates. Warning: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapors. On contact with skin, immediately wash with plenty of water.
-Dissolve 38.5 grams in 470 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Cool to 45-50°C. -Aseptically add sterile rehydrated contents of 1 vial of L.Mono Selective Supplement I and 1 vial of L.Mono Selective Supplement II. For enrichment, add sterile contents of 1 vial of L.Mono Enrichment Supplement II. -Mix well and pour into sterile Petri plates. Warning: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapors. On contact with skin, immediately wash with plenty of water.
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