L.J. MEDIUM W/O. STARCH (as per International Standard)
-Dissolve 7.24 grams in 600 ml distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organisms, addition of glycerol is not desirable).
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically.
-Add and mix egg emulsion base gently to obtain uniform mixture.
-Distribute in sterile screw capped tubes. Arrange tubes in a slanted position.
-Dissolve 7.24 grams in 600 ml distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organisms, addition of glycerol is not desirable).
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically.
-Add and mix egg emulsion base gently to obtain uniform mixture.
-Distribute in sterile screw capped tubes. Arrange tubes in a slanted position.
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