-Dissolve 57.52 grams in 1000 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Mix well and distribute into in to tubes. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Allow the tubes to cool in slanted position to form slopes with about 1inch butts, best reactions are obtained on freshly prepared medium. -Do not use screw capped tubes or bottles.
-Dissolve 57.52 grams in 1000 ml purified/distilled water. -Heat to boiling to dissolve the medium completely. -Mix well and distribute into in to tubes. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Allow the tubes to cool in slanted position to form slopes with about 1inch butts, best reactions are obtained on freshly prepared medium. -Do not use screw capped tubes or bottles.
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