-Dissolve 7.3 grams in 600 ml purified / distilled water containing 12 ml glycerol.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 1 litre of sterile whole egg emulsion, prepared under aseptic conditions.
-Mix well avoiding the formation of air bubbles and dispense in screw-capped containers. Sterilize by inspissation
at 85°C for 1 hour.
-Dissolve 7.3 grams in 600 ml purified / distilled water containing 12 ml glycerol.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 1 litre of sterile whole egg emulsion, prepared under aseptic conditions.
-Mix well avoiding the formation of air bubbles and dispense in screw-capped containers. Sterilize by inspissation
at 85°C for 1 hour.
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