ISP MEDIUM No 2 (YEAST MALT AGAR)
-Dissolve 20.5 grams in 490 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-For preparing selective media acidify the media up to pH 3.0 to 4.0 by aseptically adding 1 vial of 10% Lactic Acid
Solution, do not heat the media after addition of acid.
-Mix well and pour into sterile Petri plates.
-Dissolve 20.5 grams in 490 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-For preparing selective media acidify the media up to pH 3.0 to 4.0 by aseptically adding 1 vial of 10% Lactic Acid
Solution, do not heat the media after addition of acid.
-Mix well and pour into sterile Petri plates.
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