IRON OXIDIZING MEDIUM (DOUBLE PACK) (PART - I)
-Dissolve 4.11 grams of Part I in 1000 ml purified/distilled water containing 1 ml of 10N sulphuric acid. -Heat to boiling to dissolve the medium completely. -Dissolve 44.22 grams of Part II separately in 300 ml purified/distilled water. -Heat if necessary to dissolve the medium completely. -Sterilize Part A and Part B separately by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool each solution to 25°C. -Aseptically add 300 ml of sterilized Part B to 700 ml of Part A. Mix thoroughly. -Aseptically distribute into sterile tubes or flasks.
-Dissolve 4.11 grams of Part I in 1000 ml purified/distilled water containing 1 ml of 10N sulphuric acid. -Heat to boiling to dissolve the medium completely. -Dissolve 44.22 grams of Part II separately in 300 ml purified/distilled water. -Heat if necessary to dissolve the medium completely. -Sterilize Part A and Part B separately by autoclaving at 15 psi pressure (121°C) for 15 minutes. -Cool each solution to 25°C. -Aseptically add 300 ml of sterilized Part B to 700 ml of Part A. Mix thoroughly. -Aseptically distribute into sterile tubes or flasks.
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