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Half Fraser Broth Base (Iso 11290-1:2017)
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MLSIGNUP100
Expiring in 0days 13hr6min6sec
HALF FRASER BROTH BASE (ISO 11290-1:2017)
-Dissolve 54.92 g of the powder in 1000 mL distilled water.
-Gently heat to boiling with gentle swirling and dissolve the powder completely.
-Sterilize by autoclaving at 121°C (15 psi) for 15 minutes as per validated cycle.
-Cool to 45°C-50°C and aseptically add 2 vials of rehydrated Fraser supplement and if required, add one vial of
rehydrated Fraser Selective supplement to make the medium selective.
-Mix well and dispense as desired.
-Dissolve 54.92 g of the powder in 1000 mL distilled water.
-Gently heat to boiling with gentle swirling and dissolve the powder completely.
-Sterilize by autoclaving at 121°C (15 psi) for 15 minutes as per validated cycle.
-Cool to 45°C-50°C and aseptically add 2 vials of rehydrated Fraser supplement and if required, add one vial of
rehydrated Fraser Selective supplement to make the medium selective.
-Mix well and dispense as desired.
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